Exposure of a 23F serotype strain of Streptococcus pneumoniae to cigarette smoke condensate is associated with selective upregulation of genes encoding the two-component regulatory system 11 (TCS11)

Riana Cockeran; Jenny A Herbert; Timothy J Mitchell; Thérèse Dix-Peek; Caroline Dickens; Ronald Anderson; Charles Feldman

doi:10.1155/2014/976347

Exposure of a 23F serotype strain of Streptococcus pneumoniae to cigarette smoke condensate is associated with selective upregulation of genes encoding the two-component regulatory system 11 (TCS11)

Biomed Res Int. 2014:2014:976347. doi: 10.1155/2014/976347. Epub 2014 Jun 11.

Authors

Riana Cockeran¹, Jenny A Herbert², Timothy J Mitchell², Thérèse Dix-Peek³, Caroline Dickens³, Ronald Anderson⁴, Charles Feldman³

Affiliations

¹ Medical Research Council Unit for Inflammation and Immunity, Department of Immunology, Faculty of Health Sciences, University of Pretoria, Private Bag X323, Pretoria 0001, South Africa ; Tshwane Academic Division of the National Health Laboratory Service, 1 Modderfontein Road, Sandringham, Johannesburg 2192, South Africa.
² Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow G12 8TA, UK ; Institute for Microbiology and Infection, School of Immunity and Infection, University of Birmingham, Birmingham B15 2TT, UK.
³ Divisions of Haematology and Pulmonology, Department of Internal Medicine, Faculty of Health Sciences, University of the Witwatersrand and Charlotte Maxeke Johannesburg Academic Hospital, Private Bag 3,WITS, Johannesburg 2050, South Africa.
⁴ Medical Research Council Unit for Inflammation and Immunity, Department of Immunology, Faculty of Health Sciences, University of Pretoria, Private Bag X323, Pretoria 0001, South Africa.

Abstract

Alterations in whole genome expression profiles following exposure of the pneumococcus (strain 172, serotype 23F) to cigarette smoke condensate (160 μg/mL) for 15 and 60 min have been determined using the TIGR4 DNA microarray chip. Exposure to CSC resulted in the significant (P<0.014-0.0006) upregulation of the genes encoding the two-component regulatory system 11 (TCS11), consisting of the sensor kinase, hk11, and its cognate response regulator, rr11, in the setting of increased biofilm formation. These effects of cigarette smoke on the pneumococcus may contribute to colonization of the airways by this microbial pathogen.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / biosynthesis*
DNA-Binding Proteins / biosynthesis*
Gene Expression Regulation, Bacterial / drug effects
Histidine Kinase
Membrane Proteins / biosynthesis*
Oligonucleotide Array Sequence Analysis
Protein Kinases / biosynthesis*
Serogroup
Smoking / adverse effects*
Streptococcus pneumoniae / drug effects
Streptococcus pneumoniae / genetics*

Substances

Bacterial Proteins
DNA-Binding Proteins
Membrane Proteins
Protein Kinases
Histidine Kinase