A novel VHDJH to JH joining that induces H chain production in an Ig-null immature B cell line

J Immunol. 1989 Aug 1;143(3):1040-5.

Abstract

A novel Ig H chain gene rearrangement, a VHDJH to JH joining, was observed in an Ig-null immature B cell line. The preexisting, nonproductive VHDJH complex was replaced by the productive VHJH complex which was generated by the novel joining between the rearranged VH and a germ line JH gene. This VHDJH to JH joining is thought to be a site-specific recombinational event mediated by a putative recombination signal sequence, CACAGCC-12-base-GCAAGAAAG, embedded in the rearranged VH gene including the N region. This sequence might be a novel recombination signal sequence, which had not yet been reported, for so-called recombinase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / metabolism*
  • B-Lymphocytes / physiology
  • Base Sequence
  • Blotting, Southern
  • Cell Differentiation
  • Cell Line, Transformed
  • Cell Separation
  • Gene Rearrangement, B-Lymphocyte
  • Immunoglobulin Heavy Chains / biosynthesis*
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Joining Region / genetics*
  • Immunoglobulin Variable Region / genetics*
  • Lymphocytes, Null / metabolism*
  • Lymphocytes, Null / physiology
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data

Substances

  • Immunoglobulin Heavy Chains
  • Immunoglobulin Joining Region
  • Immunoglobulin Variable Region

Associated data

  • GENBANK/M28187
  • GENBANK/M28188
  • GENBANK/M28189
  • GENBANK/M28190