A low-cost miniaturized potentiostat for point-of-care diagnosis

Biosens Bioelectron. 2014 Dec 15;62:249-54. doi: 10.1016/j.bios.2014.06.053. Epub 2014 Jun 30.

Abstract

This paper presents a novel approach of using a miniaturized potentiostat (M-P) chip (LMP91000) to perform full range cyclic voltammetry (CV) measurements for the detection of biomarkers. The LMP91000 evaluation board was reconfigured to perform three-electrode CV measurements in order to achieve electrochemical cortisol immunosensing. The microelectrodes for cortisol estimation were fabricated by immobilizing monoclonal anti-cortisol antibody (Anti-M-Cab) onto self-assembled monolayer (SAM) modified Au microelectrodes. The results obtained using the M-P were compared to those obtained using a conventional potentiostat. The M-P was successful in measuring cortisol levels in the range of pM. The outcomes of the studies suggest that M-P can effectively perform biochemical measurements on three electrode systems, enabling the development of miniature systems for point-of-care (POC) diagnosis.

Keywords: Cortisol; Electrochemical immunosensing; Miniaturized potentiostat; Personalized health monitoring/diagnostic; Point-of-care.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Validation Study

MeSH terms

  • Antibodies, Immobilized
  • Antibodies, Monoclonal
  • Biomarkers / analysis
  • Biosensing Techniques / instrumentation*
  • Electrochemical Techniques
  • Equipment Design
  • Gold
  • Humans
  • Hydrocortisone / analysis
  • Hydrocortisone / immunology
  • Microelectrodes
  • Point-of-Care Systems*

Substances

  • Antibodies, Immobilized
  • Antibodies, Monoclonal
  • Biomarkers
  • Gold
  • Hydrocortisone