Coriander leaf extract exerts antioxidant activity and protects against UVB-induced photoaging of skin by regulation of procollagen type I and MMP-1 expression

J Med Food. 2014 Sep;17(9):985-95. doi: 10.1089/jmf.2013.2999. Epub 2014 Jul 14.


Ultraviolet (UV) radiation causes photodamage to the skin, which, in turn, leads to depletion of the dermal extracellular matrix and chronic alterations in skin structure. Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-1 (MMP-1) activity. Coriandrum sativum L. (coriander leaf, cilantro; CS) has been used as a herbal medicine for the treatment of diabetes, hyperlipidemia, liver disease, and cancer. In this study, we examined whether CS ethanol extract (CSE) has protective effects against UVB-induced skin photoaging in normal human dermal fibroblasts (NHDF) in vitro and in the skin of hairless mice in vivo. The main component of CSE, linolenic acid, was determined by gas chromatography-mass spectroscopy. We measured the cellular levels of procollagen type I and MMP-1 using ELISA in NHDF cells after UVB irradiation. NHDF cells that were treated with CSE after UVB irradiation exhibited higher procollagen type I production and lower levels of MMP-1 than untreated cells. We found that the activity of transcription factor activator protein-1 (AP-1) was also inhibited by CSE treatment. We measured the epidermal thickness, dermal collagen fiber density, and procollagen type I and MMP-1 levels in photo-aged mouse skin in vivo using histological staining and western blot analysis. Our results showed that CSE-treated mice had thinner epidermal layers and denser dermal collagen fibers than untreated mice. On a molecular level, it was further confirmed that CSE-treated mice had lower MMP-1 levels and higher procollagen type I levels than untreated mice. Our results support the potential of C. sativum L. to prevent skin photoaging.

Keywords: Coriandrum sativum; MMP-1; UVB; hairless mice; procollagen type I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use*
  • Collagen Type I / metabolism*
  • Coriandrum / chemistry*
  • Epidermis / drug effects
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Humans
  • Male
  • Matrix Metalloproteinase 1 / metabolism*
  • Mice, Hairless
  • Phytotherapy
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Plant Leaves
  • Procollagen / metabolism*
  • Skin / drug effects*
  • Skin / metabolism
  • Skin / radiation effects
  • Skin Aging / drug effects*
  • Transcription Factor AP-1 / metabolism
  • Ultraviolet Rays
  • alpha-Linolenic Acid / analysis
  • alpha-Linolenic Acid / pharmacology
  • alpha-Linolenic Acid / therapeutic use


  • Antioxidants
  • Collagen Type I
  • Plant Extracts
  • Procollagen
  • Transcription Factor AP-1
  • alpha-Linolenic Acid
  • Matrix Metalloproteinase 1