Evaluation of antidiabetic properties of cactus pear seed oil in rats

Pharm Biol. 2014 Oct;52(10):1286-90. doi: 10.3109/13880209.2014.890230. Epub 2014 Jul 15.


Context: Cactus pear (Opuntia ficus-indica (L.) Mill. (Cactaceae)) is a medicinal plant widely used to treat diabetes.

Objective: This work investigates the hypoglycemic and antihyperglycemic effect of cactus pear seed oil (CPSO), its mechanism of action, and any toxic effects.

Materials and methods: The hypoglycemic effect of CPSO was evaluated in groups of six healthy Wistar rats given 1 or 2 ml kg(-1) orally and compared with groups receiving glibenclamide (2 mg kg(-1)) or water. Glycemia was determined after 30, 60, 120, 240, and 360 min. The antihyperglycemic effect of CPSO was determined in healthy rats and in streptozotocin-induced diabetic rats (STZ); normal rats received 0.8 ml kg(-1) CPSO, while diabetic rats received 1 ml kg(-1) CPSO, their controls received water or 2 mg kg(-1) glibenclamide. For the antihyperglycemic effect evaluation, all the animals were fasted for 16 h before treatment and received glucose orally at 1 g kg(-1) 30 min after treatment; blood was taken after 30, 90, 150, and 210 min. Intestinal glucose absorption was estimated in rat jejunum perfused with a solution containing 5.55 mmol l(-1) glucose. Acute toxicity was determined in albino mice that received oral or intraperitoneal doses of 1, 3, or 5 ml kg(-1) CPSO.

Results: CPSO (p.o.) decreased postprandial hyperglycemia (60 min after glucose loading), 40.33% and 16.01%, in healthy and STZ-diabetic glucose-loaded rats, respectively. CPSO, also, significantly decreased intestinal glucose absorption by 25.42%. No adverse effects were seen in mice administered CPSO at up to 5 ml kg(-1).

Conclusion: CPSO is antihyperglycemic. The effect can be explained partly by inhibition of intestinal glucose absorption.

Keywords: Antihyperglycemic; Opuntia ficus-indica; diabetes; glucose tolerance; intestinal glucose absorption; postprandial hyperglycemia; streptozotocin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / drug effects
  • Blood Glucose / metabolism
  • Diabetes Mellitus, Experimental / blood
  • Diabetes Mellitus, Experimental / drug therapy*
  • Drug Evaluation, Preclinical / methods
  • Female
  • Hypoglycemic Agents / isolation & purification
  • Hypoglycemic Agents / pharmacology
  • Hypoglycemic Agents / therapeutic use*
  • Male
  • Mice
  • Opuntia*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Plant Oils / isolation & purification
  • Plant Oils / pharmacology
  • Plant Oils / therapeutic use*
  • Rats
  • Rats, Wistar
  • Seeds*


  • Blood Glucose
  • Hypoglycemic Agents
  • Plant Extracts
  • Plant Oils