Re-examination of the taxonomic status of Enterobacter helveticus, Enterobacter pulveris and Enterobacter turicensis as members of the genus Cronobacter and their reclassification in the genera Franconibacter gen. nov. and Siccibacter gen. nov. as Franconibacter helveticus comb. nov., Franconibacter pulveris comb. nov. and Siccibacter turicensis comb. nov., respectively

Int J Syst Evol Microbiol. 2014 Oct;64(Pt 10):3402-3410. doi: 10.1099/ijs.0.059832-0. Epub 2014 Jul 15.


Recently, a taxonomical re-evaluation of the genus Enterobacter, based on multi-locus sequence typing (MLST) analysis, has led to the proposal that the species Enterobacter pulveris, Enterobacter helveticus and Enterobacter turicensis should be reclassified as novel species of the genus Cronobacter. In the present work, new genome-scale analyses, including average nucleotide identity, genome-scale phylogeny and k-mer analysis, coupled with previously reported DNA-DNA hybridization values and biochemical characterization strongly indicate that these three species of the genus Enterobacter are not members of the genus Cronobacter, nor do they belong to the re-evaluated genus Enterobacter. Furthermore, data from this polyphasic study indicated that all three species constitute two new genera. We propose reclassifying Enterobacter pulveris and Enterobacter helveticus in the genus Franconibacter gen. nov. as Franconibacter pulveris comb. nov. (type strain 601/05(T) = LMG 24057(T) = DSM 19144(T)) and Franconibacter helveticus comb. nov. (type strain 513/05(T) = LMG 23732(T) = DSM 18396(T)), respectively, and Enterobacter turicensis in the genus Siccibacter gen. nov. as Siccibacter turicensis comb. nov. (type strain 508/05(T) = LMG 23730(T) = DSM 18397(T)).

MeSH terms

  • Bacterial Typing Techniques
  • Cronobacter / classification*
  • Cronobacter / genetics
  • DNA, Bacterial / genetics
  • Enterobacter / classification*
  • Enterobacter / genetics
  • Enterobacteriaceae / classification*
  • Enterobacteriaceae / genetics
  • Genes, Bacterial
  • Molecular Sequence Data
  • Multilocus Sequence Typing
  • Nucleic Acid Hybridization
  • Phylogeny*
  • Polymorphism, Single Nucleotide
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA


  • DNA, Bacterial
  • RNA, Ribosomal, 16S

Associated data

  • BioProject/PRJNA215839
  • BioProject/PRJNA221525
  • BioProject/PRJNA222260
  • GENBANK/AVPP00000000
  • GENBANK/AXDK00000000
  • GENBANK/AXDL00000000
  • GENBANK/AXDM00000000
  • GENBANK/AXSY00000000
  • GENBANK/AXSZ00000000