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. 2014 Jun 25;9:3131-5.
doi: 10.2147/IJN.S56439. eCollection 2014.

Lubricin as a Novel Nanostructured Protein Coating to Reduce Fibroblast Density

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Free PMC article

Lubricin as a Novel Nanostructured Protein Coating to Reduce Fibroblast Density

George Ejiofor Aninwene 2nd et al. Int J Nanomedicine. .
Free PMC article

Abstract

Excessive fibroblast adhesion and proliferation on the surface of medical implants (such as catheters, endotracheal tubes, intraocular lenses, etc) can lead to major postsurgical complications. This study showed that when coated on tissue culture polystyrene, lubricin, a nanostructured mucinous glycoprotein found in the synovial fluid of joints, decreased fibroblast density for up to 2 days of culture compared to controls treated with phosphate buffered saline (PBS). When examining why, similar antifibroblast density results were found when coating tissue culture polystyrene with bovine submaxillary mucin (BSM), an even smaller protein closely related to the central subregion of lubricin. Additionally, results from this study demonstrated that in contrast to BSM or controls (PBS-coated and non-coated samples), lubricin was better at preserving the health of nonadherent or loosely adherent fibroblasts; fibroblasts that did not adhere or loosely adhered on the lubricin-coated tissue culture polystyrene adhered and proliferated well for up to an additional day when they were reseeded on uncoated tissue culture polystyrene. In summary, this study provides evidence for the promise of nanostructured lubricin (and to a lesser extent BSM) to inhibit fibroblast adhesion and growth when coated on medical devices; lubricin should be further explored for numerous medical device applications.

Keywords: antiadhesive; fibroblasts; lubricin; mucin.

Figures

Figure 1
Figure 1
Day 1 phase contrast images of fibroblasts grown on tissue culture plates. Notes: (A) Untreated; (B) PBS-coated; (C) BSM-coated; (D) lubricin-coated. Scale bars on bottom of each image. Abbreviations: PBS, phosphate buffered saline; BSM, bovine submaxillary mucin.
Figure 2
Figure 2
Day 2 phase contrast images of fibroblasts grown on tissue culture plates. Notes: (A) Untreated; (B) PBS-coated; (C) BSM-coated; (D) lubricin-coated. Scale bars on bottom of each image. Abbreviations: PBS, phosphate buffered saline; BSM, bovine submaxillary mucin.
Figure 3
Figure 3
Day 1 phase contrast images of fibroblast aspirated and reseeded from 2 days of culture. Notes: (A) Untreated; (B) PBS-coated; (C) BSM-coated; (D) lubricin-coated tissue culture polystyrene. Cells after reseeding, cells were cultured for an additional day before images were taken. Abbreviations: PBS, phosphate buffered saline; BSM, bovine submaxillary mucin.
Figure 4
Figure 4
Decreased fibroblast density on BSM- and lubricin-coated tissue culture polystyrene as determined using the MTS assay. Notes: Original seeding density was 80,000 cells per sample. *P<0.05 comparing day 2 to day 1 on the same sample. Both lubricin- and BSM-coated wells experienced significantly lower (P<0.05) cell density compared to PBS after 1 and 2 days of culture; P<0.01 compared to PBS at the same day; P<0.01 compared to BSM at the same day. Media (BSM and lubricin) refers to media containing these respective proteins. Abbreviations: PBS, phosphate buffered saline; BSM, bovine submaxillary mucin; LUB, lubricin; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium.

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