Safety assessment of the biogenic amines in fermented soya beans and fermented bean curd

J Agric Food Chem. 2014 Aug 6;62(31):7947-54. doi: 10.1021/jf501772s. Epub 2014 Jul 25.


To evaluate the safety of biogenic amines, high performance liquid chromatography (HPLC) was used to evaluate the levels of biogenic amines in fermented soya beans and fermented bean curd. In fermented soya beans, the total biogenic amines content was in a relatively safe range in many samples, although the concentration of histamine, tyramine, and β-phenethylamine was high enough in some samples to cause a possible safety threat, and 8 of the 30 samples were deemed unsafe. In fermented bean curd, the total biogenic amines content was more than 900 mg/kg in 19 white sufu amples, a level that has been determined to pose a safety hazard; putrescine was the only one detected in all samples and also had the highest concentration, which made samples a safety hazard; the content of tryptamine, β-phenethylamine, tyramine, and histamine had reached the level of threat to human health in some white and green sufu samples, and that may imply another potential safety risk; and 25 of the 33 samples were unsafe. In conclusion, the content of biogenic amines in all fermented soya bean products should be studied and appropriate limits determined to ensure the safety of eating these foods.

MeSH terms

  • Biogenic Amines / analysis*
  • Biogenic Amines / toxicity
  • China
  • Chromatography, High Pressure Liquid
  • Fermentation*
  • Food Safety*
  • Humans
  • Maximum Allowable Concentration
  • Putrescine / analysis
  • Soy Foods / analysis*
  • Soybeans / chemistry*


  • Biogenic Amines
  • Putrescine