Differential expression of a gene highly homologous to c-ras during the development of the brine shrimp Artemia

Biochem Biophys Res Commun. 1989 Jul 31;162(2):802-8. doi: 10.1016/0006-291x(89)92381-4.


We report the identification of p21ras and a cDNA coding for it in the crustacean Artemia. The monoclonal antibody Y13-259 immunoprecipitates a polypeptide of 21.5 kDa in 24 hr-old larvae. The homology of p21ras with the Drosophila melanogaster and the mammalian p21s is in the order of 75-80% mRNA (of 1.2 kb in length) is already present in encysted gastrulae, and the levels increase, reaching a maximum before emergence. On the contrary, both the amount of p21ras and its GTP-binding activity are low prior to emergence and rises afterwards. These results suggest that p21ras expression is regulated post-transcriptionally, and that its function(s) is needed for post-hatching events, the more likely being the resumption of cell proliferation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Artemia / genetics
  • Artemia / growth & development*
  • Base Sequence
  • DNA / genetics
  • DNA / isolation & purification
  • Drosophila melanogaster / genetics
  • Gene Expression Regulation*
  • Immunosorbent Techniques
  • Larva / metabolism
  • Molecular Sequence Data
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins p21(ras)
  • Proto-Oncogenes*
  • RNA, Messenger / biosynthesis
  • Sequence Homology, Nucleic Acid


  • Proto-Oncogene Proteins
  • RNA, Messenger
  • DNA
  • Proto-Oncogene Proteins p21(ras)

Associated data

  • GENBANK/M27887