The hypusine-containing translation factor eIF5A

Crit Rev Biochem Mol Biol. Sep-Oct 2014;49(5):413-25. doi: 10.3109/10409238.2014.939608. Epub 2014 Jul 17.


In addition to the small and large ribosomal subunits, aminoacyl-tRNAs, and an mRNA, cellular protein synthesis is dependent on translation factors. The eukaryotic translation initiation factor 5A (eIF5A) and its bacterial ortholog elongation factor P (EF-P) were initially characterized based on their ability to stimulate methionyl-puromycin (Met-Pmn) synthesis, a model assay for protein synthesis; however, the function of these factors in cellular protein synthesis has been difficult to resolve. Interestingly, a conserved lysine residue in eIF5A is post-translationally modified to hypusine and the corresponding lysine residue in EF-P from at least some bacteria is modified by the addition of a β-lysine moiety. In this review, we provide a summary of recent data that have identified a novel role for the translation factor eIF5A and its hypusine modification in the elongation phase of protein synthesis and more specifically in stimulating the production of proteins containing runs of consecutive proline residues.

Keywords: Deoxyhypusine hydroxylase; deoxyhypusine synthase; elongation factor; polyproline; translation elongation.

Publication types

  • Research Support, N.I.H., Intramural
  • Review

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Humans
  • Lysine / analogs & derivatives*
  • Lysine / chemistry
  • Lysine / metabolism
  • Models, Molecular
  • Molecular Sequence Data
  • Peptide Elongation Factors / chemistry
  • Peptide Elongation Factors / metabolism
  • Peptide Initiation Factors / chemistry
  • Peptide Initiation Factors / metabolism*
  • Peptides / metabolism
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / metabolism*
  • Ribosomes / metabolism


  • Peptide Elongation Factors
  • Peptide Initiation Factors
  • Peptides
  • RNA-Binding Proteins
  • eukaryotic translation initiation factor 5A
  • factor EF-P
  • polyproline
  • hypusine
  • Lysine