Telomere-mediated chromosomal truncation in Aspergillus oryzae

Sawaki Tada; Hikaru Ohkuchi; Mayumi Matsushita-Morita; Ikuyo Furukawa; Ryota Hattori; Satoshi Suzuki; Yutaka Kashiwagi; Ken-Ichi Kusumoto

doi:10.1016/j.jbiosc.2014.06.011

Telomere-mediated chromosomal truncation in Aspergillus oryzae

J Biosci Bioeng. 2015 Jan;119(1):43-6. doi: 10.1016/j.jbiosc.2014.06.011. Epub 2014 Jul 14.

Authors

Sawaki Tada¹, Hikaru Ohkuchi¹, Mayumi Matsushita-Morita¹, Ikuyo Furukawa¹, Ryota Hattori¹, Satoshi Suzuki¹, Yutaka Kashiwagi², Ken-Ichi Kusumoto³

Affiliations

¹ National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
² Department of Fermentation Science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
³ National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. Electronic address: kusumoto@affrc.go.jp.

PMID: 25034635
DOI: 10.1016/j.jbiosc.2014.06.011

Abstract

We truncated the short arm of chromosome 3 to delete the aflatoxin biosynthesis gene homolog cluster using telomeric repeats in Aspergillus oryzae. The predicted deletion was confirmed by Southern blot analyses. This telomere-mediated chromosomal truncation method enables the development of an artificial chromosome in A. oryzae.

Keywords: Aflatoxin; Aspergillus oryzae; Chromosomal truncation; Filamentous fungi; Telomere; Telomere position effect.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aflatoxins / biosynthesis
Aspergillus oryzae / genetics*
Chromosomes, Artificial / genetics*
Chromosomes, Fungal / genetics*
Gene Deletion*
Multigene Family / genetics
Telomere / genetics*
Telomere / metabolism*

Substances

Aflatoxins