Molecular characterisation of novel mitoviruses associated with Sclerotinia sclerotiorum

Arch Virol. 2014 Nov;159(11):3157-60. doi: 10.1007/s00705-014-2171-7. Epub 2014 Jul 18.

Abstract

Seven putative mitoviral genomes, representing four species from three Sclerotinia sclerotiorum isolates, were fully sequenced. The genome lengths ranged from 2438 to 2815 nucleotides. The RNA-dependent RNA polymerase (RdRp) of one genome shared high amino acid (aa) sequence identity (98.5 %) with the previously described Sclerotinia sclerotiorum mitovirus 2 (SsMV2/NZ1) and was provisionally assigned the name SsMV2/14563. The RdRps of three of the genomes with closest aa sequence identity of 78.8-79.3 % to Sclerotinia sclerotiorum mitovirus 1 (SsMV1/KL1) were provisionally considered to represent a new species, and the corresponding virus was named Sclerotinia sclerotiorum mitovirus 5 (SsMV5/11691, SsMV5/14563 and SsMV5/Lu471). The remaining two novel genomes, for which the viruses were provisionally named Sclerotinia sclerotiorum mitovirus 6 (SsMV6/14563 and SsMV6/Lu471) and Sclerotinia sclerotiorum mitovirus 7 (SsMV7/Lu471), showed closest aa sequence identities to Sclerotinia sclerotiorum mitovirus 3 (SsMV3/NZ1; 57.5-57.8 %) and Cryphonectria cubensis mitovirus 1a (CcMV1a; 32 %), respectively. The RdRp proteins of all seven genomes contained the conserved aa sequence motifs (I-IV) previously reported for mitoviruses, and their 5' and 3' untranslated regions (UTRs) have the potential to fold into stem-loop secondary structures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascomycota / virology*
  • Molecular Sequence Data
  • Open Reading Frames
  • Phylogeny
  • Plant Diseases / microbiology*
  • RNA Replicase / chemistry
  • RNA Replicase / genetics
  • RNA Viruses / classification
  • RNA Viruses / enzymology
  • RNA Viruses / genetics*
  • RNA Viruses / isolation & purification*
  • Sequence Homology, Amino Acid
  • Viral Proteins / chemistry
  • Viral Proteins / genetics

Substances

  • Viral Proteins
  • RNA Replicase