Overexpression of TGF-β1 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cells

Biochem Biophys Res Commun. 2014 Aug 8;450(4):1593-9. doi: 10.1016/j.bbrc.2014.07.045. Epub 2014 Jul 15.


Transforming growth factor-beta (TGF-β) superfamily proteins play a critical role in proliferation, differentiation, and other functions of mesenchymal stem cells (MSCs). During chondrogenic differentiation of MSCs, TGF-β up-regulates chondrogenic gene expression by enhancing the expression of the transcription factor SRY (sex-determining region Y)-box9 (Sox9). In this study, we investigated the effect of continuous TGF-β1 overexpression in human synovium-derived MSCs (hSD-MSCs) on immunophenotype, differentiation potential, and proliferation rate. hSD-MSCs were transduced with recombinant retroviruses (rRV) encoding TGF-β1. The results revealed that continuous overexpression of TGF-β1 did not affect their phenotype as evidenced by flow cytometry and reverse transcriptase PCR (RT-PCR). In addition, continuous TGF-β1 overexpression strongly enhanced cell proliferation of hSD-MSCs compared to the control groups. Also, induction of chondrogenesis was more effective in rRV-TGFB-transduced hSD-MSCs as shown by RT-PCR for chondrogenic markers, toluidine blue staining and glycosaminoglycan (GAG)/DNA ratio. Our data suggest that overexpression of TGF-β1 positively enhances the proliferation and chondrogenic potential of hSD-MSCs.

Keywords: Chondrogenic differentiation; Proliferation; Synovium-derived mesenchymal stem cell; TGF-β.

MeSH terms

  • Cartilage / cytology*
  • Cell Differentiation*
  • Cell Proliferation*
  • Cells, Cultured
  • Flow Cytometry
  • Humans
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stem Cells / cytology*
  • Synovial Membrane / cytology*
  • Transduction, Genetic
  • Transforming Growth Factor beta1 / metabolism*


  • Transforming Growth Factor beta1