Genomic profiling of human Leishmania braziliensis lesions identifies transcriptional modules associated with cutaneous immunopathology

J Invest Dermatol. 2015 Jan;135(1):94-101. doi: 10.1038/jid.2014.305. Epub 2014 Jul 18.


The host immune response has a critical role not only in protection from human leishmaniasis but also in promoting disease severity. Although candidate gene approaches in mouse models of leishmaniasis have been extremely informative, a global understanding of the immune pathways active in lesions from human patients is lacking. To address this issue, genome-wide transcriptional profiling of Leishmania braziliensis-infected cutaneous lesions and normal skin controls was carried out. A signature of the L. braziliensis skin lesion was defined, which includes over 2,000 differentially regulated genes. Pathway-level analysis of this transcriptional response revealed key biological pathways present in cutaneous lesions, generating a testable 'metapathway' model of immunopathology and providing new insights for treatment of human leishmaniasis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adolescent
  • Adult
  • Female
  • Genomics / methods*
  • Humans
  • Inflammasomes / genetics
  • Inflammasomes / immunology
  • Leishmania braziliensis / immunology*
  • Leishmaniasis, Cutaneous* / genetics
  • Leishmaniasis, Cutaneous* / immunology
  • Leishmaniasis, Cutaneous* / parasitology
  • Male
  • Middle Aged
  • Psoriasis / genetics
  • Psoriasis / immunology
  • Skin / immunology
  • Skin / parasitology
  • Skin Ulcer* / genetics
  • Skin Ulcer* / immunology
  • Skin Ulcer* / parasitology
  • Transcription, Genetic / immunology
  • Transcriptome / immunology
  • Young Adult


  • Inflammasomes