A design for the control of apoptosis in genetically modified Saccharomyces cerevisiae

Nao Nishida; Misa Noguchi; Kouichi Kuroda; Mitsuyoshi Ueda

doi:10.1080/09168451.2014.878224

A design for the control of apoptosis in genetically modified Saccharomyces cerevisiae

Biosci Biotechnol Biochem. 2014;78(2):358-62. doi: 10.1080/09168451.2014.878224. Epub 2014 Apr 16.

Authors

Nao Nishida¹, Misa Noguchi, Kouichi Kuroda, Mitsuyoshi Ueda

Affiliation

¹ a Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.

PMID: 25036693
DOI: 10.1080/09168451.2014.878224

Abstract

We have engineered a system that holds potential for use as a safety switch in genetically modified yeasts. Human apoptotic factor BAX (no homolog in yeast), under the control of the FBP1 (gluconeogenesis enzyme) promoter, was conditionally expressed to induce yeast cell apoptosis after glucose depletion. Such systems might prove useful for the safe use of genetically modified organisms.

Keywords: Bax; Saccharomyces cerevisiae; apoptosis; genetically modified organism (GMO); gluconeogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / genetics*
Fructose-Bisphosphatase / genetics
Genetic Engineering*
Humans
Promoter Regions, Genetic / genetics
Saccharomyces cerevisiae / cytology*
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae Proteins / genetics
bcl-2-Associated X Protein / genetics

Substances

Saccharomyces cerevisiae Proteins
bcl-2-Associated X Protein
FBP1 protein, S cerevisiae
Fructose-Bisphosphatase