The quality of laboratory aspects of troponin testing in clinical practice guidelines and consensus documents needs to be improved

Clin Chim Acta. 2014 Nov 1;437:58-61. doi: 10.1016/j.cca.2014.07.006. Epub 2014 Jul 15.


Objective: The European Federation of Laboratory Medicine (EFLM) and the Union of European Medical Specialists (UEMS) joint Working Group on guidelines recently proposed a checklist to help standardize the description of laboratory investigations in clinical practice guidelines (CPG).

Methods: Nine CPGs or consensus documents published from 2011 to 2013 describing the investigation of chest pain, diagnosis of acute coronary syndrome, or myocardial infarction were evaluated against the published checklist.

Results: Clinical use of troponin analysis are commonly dealt with but the publications present variable, vague and sometimes conflicting information regarding this laboratory test being very much relied on upon making a diagnosis of acute coronary syndrome. Most of the laboratory related checklist items are not considered or need to be updated e.g. suggested analytical quality goals are not applicable for the high sensitive assays and important interferences that may lead to false positive or negative diagnoses are commonly not mentioned.

Conclusion: The current paper sums up important analytical and biological issues related to troponin assays and gives suggestions for analytical quality goals that could be included in CPG's.

Keywords: Acute coronary syndrome; Analytical quality; Guideline; Interference; Troponin.

MeSH terms

  • Clinical Laboratory Techniques / standards*
  • Clinical Laboratory Techniques / trends
  • Consensus*
  • Diagnostic Tests, Routine / standards
  • Diagnostic Tests, Routine / trends
  • Humans
  • Practice Guidelines as Topic / standards*
  • Troponin / blood*


  • Troponin