Extraction, purification and antioxidant activities of the polysaccharides from maca (Lepidium meyenii)

Shenghua Zha; Qingsheng Zhao; Jinjin Chen; Liwei Wang; Guifeng Zhang; Hong Zhang; Bing Zhao

doi:10.1016/j.carbpol.2014.05.017

Extraction, purification and antioxidant activities of the polysaccharides from maca (Lepidium meyenii)

Carbohydr Polym. 2014 Oct 13:111:584-7. doi: 10.1016/j.carbpol.2014.05.017. Epub 2014 May 21.

Authors

Shenghua Zha¹, Qingsheng Zhao², Jinjin Chen¹, Liwei Wang³, Guifeng Zhang³, Hong Zhang⁴, Bing Zhao⁵

Affiliations

¹ National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190 Beijing, PR China; Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China.
² National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190 Beijing, PR China. Electronic address: qszhao@ipe.ac.cn.
³ National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190 Beijing, PR China.
⁴ Beijing Tong Ren Tang Health Pharmaceutical Co., Ltd., PR China.
⁵ National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190 Beijing, PR China. Electronic address: bzhao@ipe.ac.cn.

PMID: 25037390
DOI: 10.1016/j.carbpol.2014.05.017

Abstract

Water-soluble polysaccharides were separated from maca (Lepidium meyenii) aqueous extract (MAE). The crude polysaccharides were deproteinized by Sevag method. During the preparation process of maca polysaccharides, amylase and glucoamylase effectively removed starch in maca polysaccharides. Four Lepidium meyenii polysaccharides (LMPs) were obtained by changing the concentration of ethanol in the process of polysaccharide precipitation. All of the LMPs were composed of rhamnose, arabinose, glucose and galactose. Antioxidant activity tests revealed that LMP-60 showed good capability of scavenging hydroxyl free radical and superoxide radical at 2.0mg/mL, the scavenging rate was 52.9% and 85.8%, respectively. Therefore, the results showed that maca polysaccharides had a high antioxidant activity and could be explored as the source of bioactive compounds.

Keywords: Antioxidant activity; Lepidium meyenii; Maca; Monosaccharide composition; Polysaccharides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antioxidants / chemistry*
Antioxidants / isolation & purification
Antioxidants / pharmacology*
Hydroxyl Radical / chemistry
Lepidium / chemistry*
Plant Extracts / chemistry*
Polysaccharides / chemistry*
Polysaccharides / isolation & purification
Polysaccharides / pharmacology*

Substances

Antioxidants
Plant Extracts
Polysaccharides
Hydroxyl Radical