Prostaglandin and thromboxane biosynthesis in isolated platelet-free human monocytes. III. The induction of cycloxygenase by colony stimulating factor-1

Prostaglandins Leukot Essent Fatty Acids. 1989 May;36(2):101-6. doi: 10.1016/0952-3278(89)90026-4.


Previous observations showing the presence in the serum of a component capable of regulating prostanoid biosynthesis in human cultured monocytes, have led us to suspect its presence in human platelets. We have purified this serum monocytotropic factor (SMF) and have shown its identity with a component of platelet membranes. Surprisingly its structure appeared to be very similar to that of a polypeptide growth factor never before identified in platelets: the colony stimulating factor-1 (CSF-1 or M-CSF). Here we show that SMF and CSF-1 have very similar biological properties. Thus, CSF-1 when released from human platelets is capable of triggering the differentiation pathway leading from blood monocytes to resident macrophages. It is likely that cycloxygenase induction plays a pivotal role in these events.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Colony-Stimulating Factors / physiology*
  • Cycloheximide / pharmacology
  • Dinoprostone / biosynthesis*
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme Induction / drug effects
  • Humans
  • In Vitro Techniques
  • Macrophage Colony-Stimulating Factor
  • Monocytes / metabolism*
  • Prostaglandin-Endoperoxide Synthases / biosynthesis*
  • Thromboxane A2 / biosynthesis*
  • Thromboxane B2 / biosynthesis


  • Colony-Stimulating Factors
  • Thromboxane B2
  • Thromboxane A2
  • Macrophage Colony-Stimulating Factor
  • Cycloheximide
  • Prostaglandin-Endoperoxide Synthases
  • Dinoprostone