Lack of effect of a P2Y6 receptor antagonist on neuropathic pain behavior in mice

Pharmacol Biochem Behav. 2014 Sep;124:389-95. doi: 10.1016/j.pbb.2014.07.009. Epub 2014 Jul 18.

Abstract

Accumulating evidence indicates that various subtypes of purinergic receptors (P2X and P2Y receptor families) play an essential role in the development and the maintenance of neuropathic pain. However, there is only limited data available about the role of P2Y6 receptors in pain processing. Here we detected P2Y6 receptor immunoreactivity in primary afferent neurons of mice and observed an upregulation in response to peripheral nerve injury. However, systemic and intrathecal administration of the P2Y6 receptor antagonist MRS2578 failed to affect the injury-induced neuropathic pain behavior. Our results suggest that P2Y6 receptors, in contrast to other purinergic receptor subtypes, are not critically involved in nerve injury-induced neuropathic pain processing in mice.

Keywords: Dorsal root ganglia; Nerve injury; Neuropathic pain; Purinergic signaling; Spinal cord.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal
  • Blotting, Western
  • Isothiocyanates / therapeutic use*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Neuralgia / drug therapy*
  • Purinergic Antagonists / therapeutic use*
  • Real-Time Polymerase Chain Reaction
  • Receptors, Purinergic P2 / drug effects*
  • Receptors, Purinergic P2 / metabolism
  • Spinal Cord / metabolism
  • Thiourea / analogs & derivatives*
  • Thiourea / therapeutic use

Substances

  • Isothiocyanates
  • N,N''-1,4-butanediylbis(N'-(3-isothiocyanatophenyl))thiourea
  • Purinergic Antagonists
  • Receptors, Purinergic P2
  • purinoceptor P2Y6
  • Thiourea