Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity

Nat Methods. 2014 Aug;11(8):817-820. doi: 10.1038/nmeth.3035. Epub 2014 Jul 20.


We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Methylation
  • Epigenesis, Genetic*
  • Genome*
  • Mice
  • Sulfites / chemistry*


  • Sulfites
  • hydrogen sulfite