miR-27a is up regulated and promotes inflammatory response in sepsis

Cell Immunol. 2014 Aug;290(2):190-5. doi: 10.1016/j.cellimm.2014.06.006. Epub 2014 Jul 10.


MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Dysregulation of miRNAs is common in sepsis. Through microRNA microarray and qRT-PCR we found that the levels of miR-27a, miR-153 and miR-143 are up regulated, while let-7a, miR-218 and miR-129-5p are down regulated in lungs of septic mice. Knocking down of miR-27a down regulates expression levels of TNF-α and IL-6 significantly via reducing the phosphorylation level of NF-κB p65 and inhibiting its DNA binding activity. Furthermore, neutralisation of miR-27a up regulates PPARγ level, down regulates TNF-α expression, relieves pulmonary inflammation and promotes survival of septic mice, which demonstrates that miR-27a plays an important role in regulating inflammatory response in sepsis and provides a potential target for clinical sepsis research and treatment.

Keywords: IL-6; Inflammatory response; NF-κB p65; Sepsis; TNF-α; miR-27a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay
  • Inflammation / genetics*
  • Inflammation / metabolism
  • Inflammation / pathology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs / biosynthesis*
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction
  • Sepsis / genetics*
  • Sepsis / metabolism
  • Sepsis / pathology*
  • Up-Regulation


  • MicroRNAs
  • Mirn27 microRNA, mouse