α-lipoic acid reduces fatty acid esterification and lipogenesis in adipocytes from overweight/obese subjects

Obesity (Silver Spring). 2014 Oct;22(10):2210-5. doi: 10.1002/oby.20846. Epub 2014 Jul 17.

Abstract

Objective: α-Lipoic acid (α-LA) is a natural occurring antioxidant with beneficial effects on obesity. The aim of this study was to investigate the putative effects of α-LA on triglyceride accumulation and lipogenesis in subcutaneous adipocytes from overweight/obese subjects and to determine the potential mechanisms involved.

Methods: Fully differentiated human subcutaneous adipocytes were treated with α-LA (100 and 250 µM) during 24 h for studying triglyceride content, de novo lipogenesis, and levels of key lipogenic enzymes. The involvement of AMP-activated protein kinase (AMPK) activation was also evaluated.

Results: α-LA down-regulated triglyceride content by inhibiting fatty acid esterification and de novo lipogenesis. These effects were mediated by reduction in fatty acid synthase (FAS), stearoyl-coenzyme A desaturase 1, and diacylglycerol O-acyltransferase 1 protein levels. Interestingly, α-LA increased AMPK and acetyl CoA carboxylase phosphorylation, while the presence of the AMPK inhibitor Compound C reversed the inhibition observed on FAS protein levels.

Conclusions: α-LA down-regulates key lipogenic enzymes, inhibiting lipogenesis and reducing triglyceride accumulation through the activation of AMPK signaling pathway in human subcutaneous adipocytes from overweight/obese subjects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / drug effects*
  • Adipocytes / metabolism
  • Adipocytes / pathology
  • Cells, Cultured
  • Down-Regulation / drug effects
  • Esterification / drug effects
  • Fatty Acids / metabolism*
  • Female
  • Humans
  • Lipid Metabolism / drug effects*
  • Lipogenesis / drug effects*
  • Obesity / metabolism*
  • Obesity / pathology
  • Overweight / metabolism*
  • Overweight / pathology
  • Subcutaneous Fat / drug effects
  • Subcutaneous Fat / metabolism
  • Subcutaneous Fat / pathology
  • Thioctic Acid / pharmacology*
  • Triglycerides / metabolism

Substances

  • Fatty Acids
  • Triglycerides
  • Thioctic Acid