Small-sided games training reduces CRP, IL-6 and leptin in sedentary, middle-aged men

Eur J Appl Physiol. 2014 Nov;114(11):2289-97. doi: 10.1007/s00421-014-2953-3. Epub 2014 Jul 22.


Purpose: Long-term physical activity is reported to improve chronic systemic inflammation, which provides protection against the ensuing development of chronic disease. Accordingly, the present study assessed changes in pro- and anti-inflammatory cytokines, aerobic capacity and body composition following 8 weeks of either small-sided games (SSG) or cycling (CYC) training compared to a sedentary control (CON) condition.

Methods: Thirty-three middle-aged, sedentary men were randomized into CYC (n = 11), SSG (n = 11), or CON (n = 11) conditions. The CYC and SSG conditions trained 3 days/week for 8 weeks, whilst CON maintained habitual activity and dietary patterns. Pre- and post-intervention testing included a dual-energy X-ray absorptiometry scan, sub-maximal (80% maximal heart rate) aerobic capacity (VO₂) and fasting venous blood. Venous blood measures for pro-inflammatory markers included C-reactive protein (CRP), interleukin (IL)-6, IL-1β, tumor necrosis factor-α, and leptin; anti-inflammatory markers included IL-10, IL-1 receptor agonist, and adiponectin.

Results: Both CYC and SSG increased submaximal power output and VO₂ (P < 0.05), decreased total body fat-mass (TB-FM; P < 0.05), and CRP (SSG, -0.45 ± 0.42 mg L(-1); P = 0.008; CYC, -0.44 ± 0.59 mg L(-1); P = 0.02). Only SSG increased total body fat-free mass (TB-FFM; +1.1 ± 1.2 kg; P = 0.001) and decreased concentration of plasma IL-6 (-0.69 ± 0.62 pg mL(-1); P = 0.002) and leptin (-2,212 ± 2,531 ng mL(-1); P = 0.014).

Conclusion: Cycling and SSG training were both effective at improving CRP, VO₂ and TB-FM. Furthermore, SSG training has also shown to be an effective training approach in reducing IL-6 and leptin and increasing muscle mass within sedentary, middle-aged men.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • C-Reactive Protein / analysis*
  • Football / physiology
  • Heart Rate
  • Humans
  • Interleukin-6 / blood*
  • Leptin / blood*
  • Male
  • Middle Aged
  • Oxygen Consumption
  • Resistance Training*
  • Sedentary Behavior*


  • Interleukin-6
  • Leptin
  • C-Reactive Protein