Pseudopelagicola Gijangensis Gen. Nov., Sp. Nov., Isolated From the Sea Squirt Halocynthia Roretzi

Int J Syst Evol Microbiol. 2014 Oct;64(Pt 10):3447-3452. doi: 10.1099/ijs.0.062067-0. Epub 2014 Jul 21.

Abstract

A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterial strain, designated YSS-7(T), was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea of South Korea. Strain YSS-7(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain YSS-7(T) clustered with the type strains of Pelagicola litoralis, Planktotalea frisia, Pacificibacter maritimus and Roseovarius marinus. Strain YSS-7(T) exhibited the highest 16S rRNA gene sequence similarity (97.7%) to the type strain of Pelagicola litoralis and sequence similarity of more than 96.0% to the type strains of some other species. Strain YSS-7(T) contained Q-10 as the predominant ubiquinone and C18:1ω7c and 11-methyl C18:1ω7c as the major fatty acids. The major polar lipids of strain YSS-7(T) were phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid and an unidentified lipid. The fatty acid and polar lipid profiles of strain YSS-7(T) were different from those of the type strains of phylogenetically related species. The DNA G+C content of strain YSS-7(T) was 55.5 mol%. Other phenotypic properties demonstrated that strain YSS-7(T) is distinguished from phylogenetically related species. On the basis of the data presented, strain YSS-7(T) is considered to represent a novel genus and species, for which the name Pseudopelagicola gijangensis gen. nov., sp. nov. is proposed. The type strain of Pseudopelagicola gijangensis is YSS-7(T) ( = KCTC 42049(T) = CECT 8540(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Molecular Sequence Data
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Rhodobacteraceae / classification*
  • Rhodobacteraceae / genetics
  • Rhodobacteraceae / isolation & purification
  • Sequence Analysis, DNA
  • Ubiquinone / analogs & derivatives
  • Ubiquinone / chemistry
  • Urochordata / microbiology*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • coenzyme Q10

Associated data

  • GENBANK/KF977839