Sphingobacterium paludis sp. nov., isolated from wetland soil

Int J Syst Evol Microbiol. 2014 Oct;64(Pt 10):3453-3458. doi: 10.1099/ijs.0.064915-0. Epub 2014 Jul 21.

Abstract

A novel Gram-stain-negative bacteria, designated S37(T), was isolated from soil of the Xixi wetland, Zhejiang province, China. Cells of strain S37(T) were aerobic, non-motile rods. Growth occurred at 10-37 °C (optimum, 25 °C), pH 5.0-9.7 (optimum, pH 7.5) and with 0-6% (w/v) NaCl (optimum, 0.5%). Based on 16S rRNA gene sequence analysis, strain S37(T) was found to be a member of the genus Sphingobacterium and shared highest similarity with Sphingobacterium composti 4M24(T) (95.78%). The major fatty acids were summed feature 3 (iso-C15:0 2-OH and/or C16:1ω7c), iso-C15:0 and iso-C17:0 3-OH, and the DNA G+C content was 43.8 mol%. The predominant respiratory quinone was MK-7. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain S37(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium paludis sp. nov. (type strain S37(T) = CGMCC 1.12801(T) = NBRC 110386(T)) is proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • China
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Molecular Sequence Data
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Sphingobacterium / classification*
  • Sphingobacterium / genetics
  • Sphingobacterium / isolation & purification
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry
  • Wetlands*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 7

Associated data

  • GENBANK/KJ150598