Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1

Yukun Huang; Xiujuan Chen; Nuo Duan; Shijia Wu; Zhouping Wang; Xinlin Wei; Yuanfeng Wang

doi:10.1016/j.foodchem.2014.06.039

Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1

Food Chem. 2015 Jan 1:166:623-629. doi: 10.1016/j.foodchem.2014.06.039. Epub 2014 Jun 21.

Authors

Yukun Huang¹, Xiujuan Chen¹, Nuo Duan¹, Shijia Wu¹, Zhouping Wang², Xinlin Wei³, Yuanfeng Wang³

Affiliations

¹ State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
² State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address: wangzp1974@hotmail.com.
³ Collage of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.

PMID: 25053102
DOI: 10.1016/j.foodchem.2014.06.039

Abstract

Enterotoxins from pathogenic bacteria are known as the main reason that can cause the bacterial foodborne diseases. In this study, aptamers that bound to Staphylococcus aureus enterotoxin C1 (SEC1) with high affinity and selectivity were generated in vitro by twelve rounds of selection based on magnetic separation technology, with a low-level dissociation constant (Kd) value of 65.14 ± 11.64 nmol/L of aptamer C10. Aptamer-based quantification of SEC1 in the food sample by a graphene oxide (GO)-based method was implemented to investigate the potential of the aptamer against SEC1 with a limit of detection of 6 ng/mL. On the basis of this work, biosensors using the selected SEC1 aptamers as new molecular recognition elements could be applied for innovative determinations of SEC1.

Keywords: Aptamer; Food; Graphene oxide; SELEX; Staphylococcus aureus enterotoxin C1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aptamers, Nucleotide / genetics*
Biosensing Techniques / methods
Enterotoxins / isolation & purification*
Food Contamination / analysis
Food Microbiology
Milk / microbiology
SELEX Aptamer Technique
Staphylococcus aureus / chemistry*

Substances

Aptamers, Nucleotide
Enterotoxins
enterotoxin C, staphylococcal