Protective effect of Nigella sativa oil against binge ethanol-induced oxidative stress and liver injury in rats

Chin J Nat Med. 2014 Jul;12(7):495-9. doi: 10.1016/S1875-5364(14)60077-7.


Aim: Nigella sativa L. (Ranunculaceae) is considered as a therapeutic plant-based medicine for liver damage. In this study, the aim was to study the effect of Nigella sativa oil (NSO) pretreatment on ethanol-induced hepatotoxicity in rats.

Method: Rats were given Nigella sativa oil at doses of 2.5 and 5.0 mL·kg(-1), orally for 3 weeks, followed by oral ethanol (EtOH) administration (5 g·kg(-1)) every 12 h three times (binge model).

Results: Binge ethanol application caused significant increases in plasma transaminase activities and hepatic triglyceride and malondialdehyde (MDA) levels. It decreased hepatic glutathione (GSH) levels, but did not change vitamins E and vitamin C levels and antioxidant enzyme activities. NSO (5.0 mL·kg(-1)) pretreatment significantly decreased plasma transaminase activities, hepatic MDA, and triglyceride levels together with amelioration in hepatic histopathological findings.

Conclusion: NSO pretreatment may be effective in protecting oxidative stress-induced hepatotoxicity after ethanol administration.

Keywords: Binge ethanol; Liver; Nigella sativa; Oxidative stress; Rats.

MeSH terms

  • Animals
  • Disease Models, Animal
  • Ethanol / adverse effects*
  • Female
  • Humans
  • Liver / drug effects
  • Liver / injuries
  • Liver / metabolism
  • Liver Diseases, Alcoholic / drug therapy*
  • Liver Diseases, Alcoholic / enzymology
  • Liver Diseases, Alcoholic / etiology
  • Liver Diseases, Alcoholic / metabolism
  • Malondialdehyde / metabolism
  • Nigella sativa / chemistry*
  • Oxidative Stress / drug effects*
  • Plant Oils / administration & dosage*
  • Protective Agents / administration & dosage*
  • Rats
  • Rats, Sprague-Dawley
  • Superoxide Dismutase / metabolism
  • Transaminases / blood


  • Plant Oils
  • Protective Agents
  • Ethanol
  • Malondialdehyde
  • caraway oil
  • Superoxide Dismutase
  • Transaminases