The protective effect of Borago Officinalis extract on amyloid β (25-35)-induced long term potentiation disruption in the dentate gyrus of male rats

Metab Brain Dis. 2015 Feb;30(1):151-6. doi: 10.1007/s11011-014-9594-4. Epub 2014 Jul 25.


Alzheimer's disease (AD) begins with impairment in synaptic functions before developing into later neurodegeneration and neural loss. In the present study we have examined the protective effects of Borago Officinalis (borage) extract on amyloid β (Aβ)--Induced long term potentiation (LTP) disruption in hippocampal dentate gyrus (DG). Wistar male rats received intrahippocampal (IHP) injection of the Aβ (25-35) and borage extract throughout gestation (100 mg/kg). LTP in perforant path- DG synapses was assessed using electrophysiology method and field excitatory post- synaptic potential (fEPSP) slope and population spike (PS) amplitude were measured by 400 Hz tetanization. Finally, the total thiol content of hippocampus was measured using colorimetric reaction based on the Ellman's method. The results showed that Aβ (25-35) significantly decreased fEPSP slope and SP amplitude comparing with the control and sham group, whereas borage extract administration increased these parameters compared to the Aβ group. Aβ induced a remarkable decrease in total thiol content of hippocampus and borage prevented the decrease of the hippocampal total sulfhydryl (SH) groups. This data suggest that Aβ (25-35) can effectively inhibit LTP in the granular cells of the DG in hippocampus, and borage supplementation reverse the synaptic plasticity in DG following Aβ treatment and that borage consumption may lead to an improvement of AD-induced cognitive dysfunction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid beta-Peptides / toxicity*
  • Animals
  • Antioxidants / pharmacology*
  • Borago / chemistry*
  • Dentate Gyrus / drug effects*
  • Drug Evaluation, Preclinical
  • Hippocampus / chemistry
  • Hippocampus / drug effects
  • Injections
  • Long-Term Potentiation / drug effects*
  • Male
  • Memory Disorders / chemically induced
  • Memory Disorders / drug therapy*
  • Peptide Fragments / toxicity*
  • Phytotherapy
  • Plant Extracts / pharmacology*
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Sulfhydryl Compounds / analysis


  • Amyloid beta-Peptides
  • Antioxidants
  • Peptide Fragments
  • Plant Extracts
  • Sulfhydryl Compounds
  • amyloid beta-protein (25-35)