Zero-mode waveguide detection of flow-driven DNA translocation through nanopores

Phys Rev Lett. 2014 Jul 11;113(2):028302. doi: 10.1103/PhysRevLett.113.028302. Epub 2014 Jul 9.

Abstract

We directly measure the flow-driven injection of DNA through nanopores at the level of single molecule and single pore using a modified zero-mode waveguide method. We observe a flow threshold independent of the pore radius, the DNA concentration, and length. We demonstrate that the flow injection of DNA in nanopores is controlled by an energy barrier as proposed in the de Gennes-Brochard suction model. Finally, we show that the height of the energy barrier is modulated by functionalizing the nanopores.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage lambda / genetics
  • Benzoxazoles / chemistry
  • DNA / chemistry*
  • DNA, Viral / chemistry
  • Flow Injection Analysis / methods*
  • Fluorescent Dyes / chemistry
  • Intercalating Agents / chemistry
  • Models, Chemical*
  • Nanopores*
  • Quinolinium Compounds / chemistry
  • Structure-Activity Relationship
  • Thermodynamics

Substances

  • Benzoxazoles
  • DNA, Viral
  • Fluorescent Dyes
  • Intercalating Agents
  • Quinolinium Compounds
  • 1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium
  • DNA