Homing and migration assays of hematopoietic stem/progenitor cells

Methods Mol Biol. 2014;1185:279-84. doi: 10.1007/978-1-4939-1133-2_19.

Abstract

Hematopoietic stem and progenitor cells (HSPCs) reside mainly in bone marrow; however, under homeostatic and stressed conditions, HSPCs dynamically change their location-either egressing from bone marrow and getting into circulation, a process of mobilization; or coming back to the bone marrow, the homing process. How to analyze these two processes will be critical for understanding the behavior of HSPCs. Here we provide an experimental protocol to monitor and analyze homing and migration of HSPCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD34 / metabolism
  • Bone Marrow Cells / cytology
  • Cell Migration Assays / methods*
  • Flow Cytometry
  • Fluoresceins / metabolism
  • Hematopoietic Stem Cell Mobilization*
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / metabolism
  • Membrane Proteins / metabolism
  • Mice
  • Molecular Imaging
  • Proto-Oncogene Proteins pp60(c-src) / metabolism
  • Succinimides / metabolism

Substances

  • 5-(6)-carboxyfluorescein diacetate succinimidyl ester
  • Antigens, CD34
  • Fluoresceins
  • Membrane Proteins
  • Succinimides
  • flt3 ligand protein
  • Proto-Oncogene Proteins pp60(c-src)