Application of enzyme/zeolite sensor for urea analysis in serum

Mater Sci Eng C Mater Biol Appl. 2014 Sep;42:155-60. doi: 10.1016/j.msec.2014.05.028. Epub 2014 May 22.

Abstract

Urea biosensor based on zeolite-adsorbed urease was applied for analysis of blood serum samples. It should be noted, that this biosensor has a number of advantages, such as simple and fast performance, the absence of toxic compounds during biosensor preparation, high reproducibility and repeatability (RSD=9% and 4%, respectively). The linear range of urea determination by using the biosensor was 0.003-0.75 mM, and the limit of urea detection was 3 μM. The method of standard addition was used for analysis of serum samples with 500-fold dilution. Total time of analysis was 10 min. Good reproducibility of urea determination in real samples was demonstrated (RSD=10%). Biosensor results were verified by using a common method of urea determination (diacetyl monoxime reaction). It was shown that by using this biosensor distinguishing healthy people from people with renal dysfunction becomes easier.

Keywords: Biosensor; Conductometric transducer; Enzyme adsorption; Silicalite; Urease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • Enzymes, Immobilized / metabolism
  • Humans
  • Limit of Detection
  • Linear Models
  • Reproducibility of Results
  • Urea / blood*
  • Urea / chemistry
  • Urea / metabolism
  • Urease / metabolism*
  • Zeolites / chemistry*

Substances

  • Enzymes, Immobilized
  • Zeolites
  • Urea
  • Urease