Delta-sleep inducing peptide entrapment in the charged macroporous matrices

Mater Sci Eng C Mater Biol Appl. 2014 Sep:42:461-5. doi: 10.1016/j.msec.2014.05.059. Epub 2014 Jun 6.

Abstract

Various biomolecules, for example proteins, peptides etc., entrapped in polymer matrices, impact interactions between matrix and cells, including stimulation of cell adhesion and proliferation. Delta-sleep inducing peptide (DSIP) possesses numerous beneficial properties, including its abilities in burn treatment and neuronal protection. DSIP entrapment in two macroporous polymer matrices based on copolymer of dimethylaminoethyl methacrylate and methylen-bis-acrylamide (Co-DMAEMA-MBAA) and copolymer of acrylic acid and methylen-bis-acrylamide (Co-AA-MBAA) has been studied. Quite 100% of DSIP has been entrapped into positively charged Co-DMAEMA-MBAA matrix, while the quantity of DSIP adsorbed on negatively charged Co-AA-MBAA was only 2-6%. DSIP release from Co-DMAEMA-MBAA was observed in saline solutions (0.9% NaCl and PBS) while there was no DSIP release in water or 25% ethanol, thus ionic strength was a reason of this process.

Keywords: Adsorption; Biopolymers; Delta-sleep inducing peptide; Drug delivery systems; Macroporous polymers.

MeSH terms

  • Acrylamides / chemistry*
  • Adsorption
  • Delta Sleep-Inducing Peptide / chemistry
  • Delta Sleep-Inducing Peptide / isolation & purification*
  • Delta Sleep-Inducing Peptide / pharmacokinetics*
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Hydrogen-Ion Concentration
  • Materials Testing
  • Methacrylates / chemistry*
  • Polymers / chemistry*
  • Porosity
  • Sodium Chloride

Substances

  • Acrylamides
  • Methacrylates
  • Polymers
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Sodium Chloride
  • Delta Sleep-Inducing Peptide
  • N,N'-methylenebisacrylamide
  • 2-(dimethylamino)ethyl methacrylate