Crucial role of the Rcl1p-Bms1p interaction for yeast pre-ribosomal RNA processing

Nucleic Acids Res. 2014 Sep;42(15):10161-72. doi: 10.1093/nar/gku682. Epub 2014 Jul 26.


The essential Rcl1p and Bms1p proteins form a complex required for 40S ribosomal subunit maturation. Bms1p is a GTPase and Rcl1p has been proposed to catalyse the endonucleolytic cleavage at site A2 separating the pre-40S and pre-60S maturation pathways. We determined the 2.0 Å crystal structure of Bms1p associated with Rcl1p. We demonstrate that Rcl1p nuclear import depends on Bms1p and that the two proteins are loaded into pre-ribosomes at a similar stage of the maturation pathway and remain present within pre-ribosomes after cleavage at A2. Importantly, GTP binding to Bms1p is not required for the import in the nucleus nor for the incorporation of Rcl1p into pre-ribosomes, but is essential for early pre-rRNA processing. We propose that GTP binding to Bms1p and/or GTP hydrolysis may induce conformational rearrangements within the Bms1p-Rcl1p complex allowing the interaction of Rcl1p with its RNA substrate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Cell Nucleus / metabolism
  • GTP-Binding Proteins / chemistry*
  • GTP-Binding Proteins / metabolism*
  • Gene Expression Regulation, Fungal
  • Guanosine Triphosphate / metabolism
  • Nuclear Proteins / chemistry*
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Point Mutation
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • RNA Precursors / metabolism
  • RNA Processing, Post-Transcriptional*
  • RNA, Ribosomal / metabolism*
  • RNA-Binding Proteins / chemistry*
  • RNA-Binding Proteins / metabolism*
  • Ribosomes / metabolism
  • Saccharomyces cerevisiae Proteins / chemistry*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*


  • Bms1 protein, S cerevisiae
  • Nuclear Proteins
  • RCL1 protein, S cerevisiae
  • RNA Precursors
  • RNA, Ribosomal
  • RNA-Binding Proteins
  • Saccharomyces cerevisiae Proteins
  • Guanosine Triphosphate
  • GTP-Binding Proteins

Associated data

  • PDB/4CLQ