Multidimensional profiling in the investigation of small-molecule-induced cell death

Adam J Wolpaw; Brent R Stockwell

doi:10.1016/B978-0-12-801430-1.00011-1

Multidimensional profiling in the investigation of small-molecule-induced cell death

Methods Enzymol. 2014;545:265-302. doi: 10.1016/B978-0-12-801430-1.00011-1.

Authors

Adam J Wolpaw¹, Brent R Stockwell²

Affiliations

¹ Residency Program in Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
² Department of Biological Sciences, Columbia University, New York, USA; Department of Chemistry, Columbia University, New York, USA; Howard Hughes Medical Institute, Columbia University, New York, USA. Electronic address: bstockwell@columbia.edu.

PMID: 25065894
DOI: 10.1016/B978-0-12-801430-1.00011-1

Abstract

Numerous morphological variations of cell death have been described. These processes depend on a complex and overlapping cellular signaling network, making molecular definition of the pathways challenging. This review describes one solution to this problem for small-molecule-induced death, the creation of high-dimensionality profiles for compounds that can be used to define and compare pathways. Such profiles have been assembled from gene expression measurements, protein quantification, chemical-genetic interactions, chemical combination interactions, cancer cell line sensitivity profiling, quantitative imaging, and modulatory profiling. We discuss the advantages and limitations of these techniques in the study of cell death.

Keywords: Apoptosis; Cell death; Chemical biology; Gene expression; Nonapoptotic cell death; Profiling; Small molecules.

Publication types

Review

MeSH terms

Apoptosis / drug effects*
Cell Death / drug effects*
Gene Expression Profiling / methods*
Humans
Signal Transduction / drug effects
Small Molecule Libraries / administration & dosage
Small Molecule Libraries / chemistry

Substances

Small Molecule Libraries

Grant support

R01 CA097061/CA/NCI NIH HHS/United States