A chemically standardized extract of Ziziphus jujuba fruit (Jujube) stimulates expressions of neurotrophic factors and anti-oxidant enzymes in cultured astrocytes

Phytother Res. 2014 Nov;28(11):1727-30. doi: 10.1002/ptr.5202. Epub 2014 Jul 28.


The fruit of Ziziphus jujuba Mill., known as jujube or Chinese date, is commonly consumed as a health supplement worldwide. To study the role of jujube in brain benefits, the expression of neurotrophic factors and anti-oxidant enzymes in the jujube-treated cultured astrocytes was determined. Application of a chemical standardized water extract of jujube in cultured astrocytes for 24 h stimulated the expressions of nerve growth factor, brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor in a concentration-dependent manner. The pre-treatment with H89, a protein kinase A inhibitor, attenuated the jujube-induced expression of neurotrophic factors. In parallel, the treatment of jujube water extract induced the transcriptional expressions of the enzymes responsible for anti-oxidation, i.e.

Nad(p)h: quinine oxidoreductase 1, glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and glutathione S-transferase, in a concentration-dependent manner. These results proposed the benefits of jujube in regulating expressions of neurotrophic factors and anti-oxidant enzymes in cultured astrocytes.

Keywords: Rhamnaceae; Ziziphus jujuba; anti-oxidant enzyme; neurotrophic factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism*
  • Astrocytes / drug effects*
  • Astrocytes / enzymology
  • Brain-Derived Neurotrophic Factor / metabolism
  • Cells, Cultured
  • Fruit / chemistry
  • Glial Cell Line-Derived Neurotrophic Factor / metabolism
  • Glutamate-Cysteine Ligase / metabolism
  • Glutathione Transferase / metabolism
  • NAD(P)H Dehydrogenase (Quinone) / metabolism
  • Nerve Growth Factor / metabolism
  • Nerve Growth Factors / metabolism*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Rats, Sprague-Dawley
  • Ziziphus / chemistry*


  • Antioxidants
  • Brain-Derived Neurotrophic Factor
  • Glial Cell Line-Derived Neurotrophic Factor
  • Nerve Growth Factors
  • Plant Extracts
  • Nerve Growth Factor
  • NAD(P)H Dehydrogenase (Quinone)
  • NQO1 protein, rat
  • Glutathione Transferase
  • GCLM protein, rat
  • Glutamate-Cysteine Ligase
  • GCLC protein, rat