Structural basis for AMPK activation: natural and synthetic ligands regulate kinase activity from opposite poles by different molecular mechanisms

Structure. 2014 Aug 5;22(8):1161-1172. doi: 10.1016/j.str.2014.06.009. Epub 2014 Jul 24.

Abstract

AMP-activated protein kinase (AMPK) is a principal metabolic regulator affecting growth and response to cellular stress. Comprised of catalytic and regulatory subunits, each present in multiple forms, AMPK is best described as a family of related enzymes. In recent years, AMPK has emerged as a desirable target for modulation of numerous diseases, yet clinical therapies remain elusive. Challenges result, in part, from an incomplete understanding of the structure and function of full-length heterotrimeric complexes. In this work, we provide the full-length structure of the widely expressed α1β1γ1 isoform of mammalian AMPK, along with detailed kinetic and biophysical characterization. We characterize binding of the broadly studied synthetic activator A769662 and its analogs. Our studies follow on the heels of the recent disclosure of the α2β1γ1 structure and provide insight into the distinct molecular mechanisms of AMPK regulation by AMP and A769662.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • AMP-Activated Protein Kinases / chemistry*
  • AMP-Activated Protein Kinases / metabolism
  • AMP-Activated Protein Kinases / physiology*
  • Adenosine Monophosphate / metabolism
  • Allosteric Site / genetics
  • Drug Delivery Systems
  • Enzyme Activation / physiology*
  • Humans
  • Kinetics
  • Ligands
  • Models, Molecular*
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Phosphorylation
  • Protein Conformation
  • Protein Isoforms / chemistry
  • Protein Isoforms / metabolism
  • Protein Isoforms / physiology
  • Pyrones / metabolism
  • Structure-Activity Relationship
  • Surface Plasmon Resonance
  • Thiophenes / metabolism

Substances

  • A 769662
  • Ligands
  • Protein Isoforms
  • Pyrones
  • Thiophenes
  • Adenosine Monophosphate
  • AMP-Activated Protein Kinases

Associated data

  • PDB/4QFG
  • PDB/4QFR
  • PDB/4QFS