Reusable and robust high sensitive non-enzymatic glucose sensor based on Ni(OH)₂ nanoparticles

Anal Chim Acta. 2014 Aug 11;839:26-33. doi: 10.1016/j.aca.2014.06.016. Epub 2014 Jun 13.


Nickel hydroxide nanoparticles were successfully electrodeposited on graphite electrode (Gr/NiONP) and employed as a robust non-enzymatic glucose sensor. The results of cyclic voltammetry (CV) and chronoamperometry demonstrated that the Gr/NiONP electrode displayed high electrocatalytic activity toward glucose. The oxidation current is directly related to the glucose concentration from 1 μM to 15 mM. Besides, the glucose sensor displayed high sensitivity (2400 μA mM(-1) cm(-2)) with a detection limit of 0.53 μM (S/N=3) in basic solution. Moreover, the sensor showed excellent selectivity, reproducibility and stability properties. The relative standard deviation is 1.2% for 10 successive measurements in 16 μM glucose. Interestingly, the signal for glucose was maintained at 95% of its initial value even after 6 months of storage under ambient conditions. Gr/NiONP electrode has also been tested to detect glucose in human serum with satisfactory results.

Keywords: Glucose; Graphite; Nanoparticle; Nickel hydroxide; Non-enzymatic sensor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques*
  • Glucose / analysis*
  • Hydroxides / chemistry*
  • Microscopy, Electron, Scanning
  • Nanoparticles*
  • Nickel / chemistry*
  • Oxidation-Reduction
  • Reproducibility of Results
  • X-Ray Diffraction


  • Hydroxides
  • nickel hydroxide
  • Nickel
  • Glucose