Collagen-based engineered heart muscle

Methods Mol Biol. 2014:1181:167-76. doi: 10.1007/978-1-4939-1047-2_15.


Cardiac muscle engineering has evolved over nearly 20 years from a scientific oddity to a mainstream technology with a wide range of applications. Of the many published methods it appears that hydrogels constitute the preferred scaffolds for myocardial tissue engineering and support of organotypic development. Here we describe a simple and highly robust protocol for the generation of engineered heart muscle using a collagen-based hydrogel method.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Collagen Type I / chemistry
  • Collagen Type I / pharmacology*
  • Humans
  • Muscle Contraction / drug effects
  • Myocardium / cytology*
  • Quality Control
  • Tissue Engineering / methods*


  • Collagen Type I