The X gene of hepatitis B virus induced growth stimulation and tumorigenic transformation of mouse NIH3T3 cells

Jpn J Cancer Res. 1989 Jul;80(7):617-21. doi: 10.1111/j.1349-7006.1989.tb01686.x.


To examine the transforming potential of the X gene product of hepatitis B virus (HBV), the X-gene-containing region (referred to as the HBx region) was introduced into mouse NIH3T3 cells. Each transformed cell line expressed X-coding mRNA at a different level. A positive correlation was found between the level of X-coding mRNA and the saturation density of the cells. The HBx-transformed cell lines exhibited X protein production and tumor formation in nude mice. The function of HBV in oncogenesis may involve the continuous expression of the X-gene-coded product in the HBV DNA-integrated cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Line
  • Cell Transformation, Viral*
  • Cloning, Molecular
  • Genes, Viral*
  • Hepatitis B virus / genetics*
  • Mice
  • Mice, Nude
  • Neoplasms, Experimental / microbiology*
  • Plasmids
  • Viral Structural Proteins / genetics*


  • Viral Structural Proteins