Abstract
A transcriptional fusion of the recE gene to a reporter gene has been constructed. Expression of recE was found to be induced upon damage to DNA with either mitomycin C or nalidixic acid. This specific transcriptional induction is blocked by a recE mutation. Mutations affecting the recB, recF and recL gene products markedly reduced induction. However, derepression of recE seems to be independent of the ATP-dependent DNase activity of the exonuclease V enzyme (also called AddAB enzyme).
MeSH terms
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Bacillus subtilis / enzymology*
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Bacillus subtilis / genetics
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DNA Damage
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DNA Repair / physiology*
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Escherichia coli Proteins*
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Exodeoxyribonucleases / biosynthesis*
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Exodeoxyribonucleases / genetics
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Gene Expression Regulation, Bacterial
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Mitomycin
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Mitomycins / pharmacology
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Mutation
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Nalidixic Acid / pharmacology
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Plasmids
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Recombinant Fusion Proteins / genetics
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Recombination, Genetic / genetics*
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SOS Response, Genetics / physiology*
Substances
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Escherichia coli Proteins
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Mitomycins
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Recombinant Fusion Proteins
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Nalidixic Acid
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Mitomycin
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Exodeoxyribonucleases
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recE protein, E coli