Evaluation of MODS assay for rapid detection of Mycobacterium tuberculosis resistance to second-line drugs in a tertiary care tuberculosis hospital in China

Tuberculosis (Edinb). 2014 Sep;94(5):506-10. doi: 10.1016/j.tube.2014.06.005. Epub 2014 Jul 16.

Abstract

In this study we evaluated the performance of microscopic observation drug susceptibility (MODS) assay for rapid detection of Mycobacterium tuberculosis resistance to second-line drugs. 246 multidrug-resistant M. tuberculosis clinical isolates were used to compare MODS with the agar proportion method for rapid detection of resistance to 8 second-line drugs: ofloxacin, amikacin, kanamycin, capreomycin, ethionamide, cycloserine, ciprofloxacin and para-aminosalicylic acid. The sensitivity of the MODS for different drugs ranged from 88.1% to 100%, whereas the specificity ranged from 92.3% to 100%. Results for MODS assay were obtained in a median time of 7 days (range 5-18). Thus MODS assay could be used as a fast, reliable and inexpensive method for detection of M. tuberculosis resistance to second-line drugs in resource-limited settings.

Keywords: Drug resistance; MODS; Mycobacterium tuberculosis; Second-line drugs.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antitubercular Agents / therapeutic use*
  • China
  • Drug Resistance, Multiple, Bacterial* / genetics
  • Humans
  • Microbial Sensitivity Tests / methods*
  • Microscopy*
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / growth & development
  • Mycobacterium tuberculosis / isolation & purification
  • Polymerase Chain Reaction
  • Predictive Value of Tests
  • Reproducibility of Results
  • Tertiary Care Centers*
  • Time Factors
  • Tuberculosis, Multidrug-Resistant / diagnosis*
  • Tuberculosis, Multidrug-Resistant / drug therapy
  • Tuberculosis, Multidrug-Resistant / microbiology

Substances

  • Antitubercular Agents