Nucleotide diversity of Maize ZmBT1 gene and association with starch physicochemical properties

Shuhui Xu; Zefeng Yang; Enying Zhang; Ying Jiang; Liang Pan; Qing Chen; Zhengwen Xie; Chenwu Xu

doi:10.1371/journal.pone.0103627

Nucleotide diversity of Maize ZmBT1 gene and association with starch physicochemical properties

PLoS One. 2014 Aug 1;9(8):e103627. doi: 10.1371/journal.pone.0103627. eCollection 2014.

Authors

Shuhui Xu¹, Zefeng Yang¹, Enying Zhang², Ying Jiang¹, Liang Pan¹, Qing Chen¹, Zhengwen Xie¹, Chenwu Xu¹

Affiliations

¹ Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, China.
² Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, China; College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, Shandong, China.

Abstract

Cereal Brittle1 protein has been demonstrated to be involved in the ADP-Glc transport into endosperm plastids, and plays vital roles in the biosynthesis of starch. In this study, the genomic sequences of the ZmBT1 gene in 80 elite maize inbred lines were obtained, and the nucleotide polymorphisms and haplotype diversity were detected. A total of 30 variants, including 22 SNPs and 8 indels, were detected from the full sequences of this gene. Among these polymorphic sites, 9 SNPs and 2 indels were found to be located in the coding region. The polymorphisms of CDS sequences classified the maize ZmBT1 gene into 6 haplotypes, which encode 6 different ZmBT1 proteins. Neutrality tests revealed a decrease in population size and/or balancing selection on the maize ZmBT1 locus. To detect the association between sequence variations of this gene and the starch physicochemical properties, 7 pasting and 4 gelatinization traits of starch were measured for the tested inbred lines using rapid visco analyzer (RVA) and differential scanning calorimeter (DSC), respectively. The result of association analysis revealed that an indel in the coding region was significantly associated with the phenotypic variation of starch gelatinization enthalpy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Endosperm / metabolism
Genetic Variation / genetics
Haplotypes / genetics
Plant Proteins / genetics
Plant Proteins / metabolism*
Polymorphism, Single Nucleotide / genetics
Starch / metabolism*
Zea mays / genetics*
Zea mays / metabolism*

Substances

Plant Proteins
Starch

Grants and funding

This work was supported by grants from the National Training Programs of Innovation and Entrepreneurship for Undergraduates (201311117035Z). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.