Oxidative stress activates AMPK in cultured cells primarily by increasing cellular AMP and/or ADP

FEBS Lett. 2014 Sep 17;588(18):3361-6. doi: 10.1016/j.febslet.2014.07.025. Epub 2014 Jul 30.

Abstract

AMPK is known to be activated by oxidative stress. Addition of glucose oxidase to cells generates H2O2 at a constant rate that is opposed by enzymic degradation, providing a good model for physiological oxidative stress. AMPK activation by glucose oxidase correlated with increases in cellular AMP:ATP and was greatly reduced in cells expressing an AMP-insensitive AMPK mutant, although a small degree of activation remained. The effects of increased AMP were partly due to inhibition of Thr172 dephosphorylation. These results suggest that changes in adenine nucleotides, rather than direct oxidative modification, are the major drivers of AMPK activation during oxidative stress.

Keywords: ADP; AMP; AMP-activated protein kinase; Hydrogen peroxide; Oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AMP-Activated Protein Kinases / metabolism*
  • Adenosine Diphosphate / metabolism*
  • Adenosine Monophosphate / metabolism*
  • Enzyme Activation
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Hydrogen Peroxide / metabolism
  • Oxidative Stress*
  • Protein Processing, Post-Translational

Substances

  • Adenosine Monophosphate
  • Adenosine Diphosphate
  • Hydrogen Peroxide
  • AMP-Activated Protein Kinases