Abstract
A strain of thermophilic fungus, Thermoascus aurantiacus, was isolated from local soil. From the culture filtrates of the organism grown on blotting paper, a xylanase, beta-glucosidase, exocellulase, and endocellulase were obtained in large amounts in highly purified form by employing ion-exchange and gel-permeation chromatography. The xylanase was crystallized. The xylanase and endocellulase were stable at 70 degrees C for 8 h, whereas the beta-glucosidase and exocellulase were less stable at 70 degrees C.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Ascomycota / enzymology*
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Ascomycota / growth & development
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Carbohydrates / analysis
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Carbon
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Cellulose 1,4-beta-Cellobiosidase
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Crystallization
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Electrophoresis, Polyacrylamide Gel
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Enzyme Stability
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Extracellular Space / enzymology
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Fungal Proteins / biosynthesis
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Fungal Proteins / isolation & purification*
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Glucosidases / isolation & purification*
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Glycoside Hydrolases / biosynthesis
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Glycoside Hydrolases / isolation & purification*
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Hot Temperature
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Xylan Endo-1,3-beta-Xylosidase
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beta-Glucosidase / biosynthesis
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beta-Glucosidase / isolation & purification*
Substances
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Carbohydrates
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Fungal Proteins
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Carbon
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Glucosidases
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Glycoside Hydrolases
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beta-Glucosidase
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Xylan Endo-1,3-beta-Xylosidase
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Cellulose 1,4-beta-Cellobiosidase