Objective: To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies.
Materials and methods: Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.
Results: Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.
Conclusion: We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.
Keywords: Adenoid cystic carcinoma; Cell line; Salivary gland tumors.
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