Development and characterization of salivary adenoid cystic carcinoma cell line

Oral Oncol. 2014 Oct;50(10):991-9. doi: 10.1016/j.oraloncology.2014.06.012. Epub 2014 Jul 30.


Objective: To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies.

Materials and methods: Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.

Results: Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.

Conclusion: We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.

Keywords: Adenoid cystic carcinoma; Cell line; Salivary gland tumors.

Publication types

  • Case Reports
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Biomarkers, Tumor / metabolism
  • Carcinoma, Adenoid Cystic / genetics
  • Carcinoma, Adenoid Cystic / metabolism
  • Carcinoma, Adenoid Cystic / pathology*
  • Cell Line, Tumor
  • Culture Media, Serum-Free
  • DNA Primers
  • Humans
  • Karyotyping
  • Male
  • Mice
  • Mice, Nude
  • Microsatellite Repeats
  • Middle Aged
  • Reverse Transcriptase Polymerase Chain Reaction
  • Salivary Gland Neoplasms / genetics
  • Salivary Gland Neoplasms / metabolism
  • Salivary Gland Neoplasms / pathology*
  • Telomerase / genetics


  • Biomarkers, Tumor
  • Culture Media, Serum-Free
  • DNA Primers
  • TERT protein, human
  • Telomerase