TopBP1: A BRCT-scaffold protein functioning in multiple cellular pathways

DNA Repair (Amst). 2014 Oct;22:165-74. doi: 10.1016/j.dnarep.2014.06.004. Epub 2014 Jul 30.


Human TopBP1 contains nine BRCT domains and functions in DNA replication initiation, checkpoint signalling, DNA repair and influences transcriptional control. TopBP1 and its homologues have been the subject of numerous scientific publications since the last comprehensive review in 2005, emerging as a key scaffold protein that links crucial components within these distinct cellular processes. This review focuses on recently published work, with particular emphasis on structural insights into TopBP1 function and the binding partners identified for DNA replication initiation, DNA-dependent checkpoints, DNA repair and transcription. We further summarise what is known about TopBP1 and links to human disease.

Keywords: Scaffold proteins; TopBP1 functions in checkpoint signalling; TopBP1 functions in replication; TopBP1 review.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Cycle Checkpoints
  • DNA Repair*
  • DNA Replication
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Humans
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Protein Structure, Tertiary


  • Carrier Proteins
  • DNA-Binding Proteins
  • Nuclear Proteins
  • TOPBP1 protein, human