Impact of extraction parameters on the recovery of lipolytic activity from fermented babassu cake

PLoS One. 2014 Aug 4;9(8):e103176. doi: 10.1371/journal.pone.0103176. eCollection 2014.

Abstract

Enzyme extraction from solid matrix is as important step in solid-state fermentation to obtain soluble enzymes for further immobilization and application in biocatalysis. A method for the recovery of a pool of lipases from Penicillium simplicissimum produced by solid-state fermentation was developed. For lipase recovery different extraction solution was used and phosphate buffer containing Tween 80 and NaCl showed the best results, yielding lipase activity of 85.7 U/g and 65.7 U/g, respectively. The parameters with great impacts on enzyme extraction detected by the Plackett-Burman analysis were studied by Central Composite Rotatable experimental designs where a quadratic model was built showing maximum predicted lipase activity (160 U/g) at 25°C, Tween 80 0.5% (w/v), pH 8.0 and extraction solution 7 mL/g, maintaining constant buffer molarity of 0.1 M and 200 rpm. After the optimization process a 2.5 fold increase in lipase activity in the crude extract was obtained, comparing the intial value (64 U/g) with the experimental design (160 U/g), thus improving the overall productivity of the process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Arecaceae / metabolism*
  • Complex Mixtures
  • Electrophoresis, Polyacrylamide Gel
  • Fermentation*
  • Hydrogen-Ion Concentration
  • Kinetics
  • Lipase / isolation & purification*
  • Lipolysis*
  • Penicillium / enzymology*
  • Solutions
  • Solvents
  • Temperature
  • Time Factors
  • Waste Products*

Substances

  • Complex Mixtures
  • Solutions
  • Solvents
  • Waste Products
  • Lipase

Grant support

This work was financially supported by the PETROBRAS and the Brazilian governmental funding agencies CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and FAPERJ (Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.