Recent Advances in Genomic DNA Sequencing of Microbial Species From Single Cells

Nat Rev Genet. 2014 Sep;15(9):577-84. doi: 10.1038/nrg3785. Epub 2014 Aug 5.


The vast majority of microbial species remain uncultivated and, until recently, about half of all known bacterial phyla were identified only from their 16S ribosomal RNA gene sequence. With the advent of single-cell sequencing, genomes of uncultivated species are rapidly filling in unsequenced branches of the microbial phylogenetic tree. The wealth of new insights gained from these previously inaccessible groups is providing a deeper understanding of their basic biology, taxonomy and evolution, as well as their diverse roles in environmental ecosystems and human health.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacteria / classification
  • Bacteria / genetics*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Evolution, Molecular
  • Genetic Variation
  • Genome, Bacterial / genetics*
  • Genomics*
  • Microbiota*
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA / methods*


  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S