Fluorescence and labelling: how to choose and what to do

Exp Suppl. 2014;105:1-24. doi: 10.1007/978-3-0348-0856-9_1.

Abstract

This chapter provides an overview of fluorescent labelling of different reactants related to the biochemistry of motor proteins. The fluorescent properties of different labels and the advantages and disadvantages of the labelling methods are discussed. This will allow for a careful selection of fluorescent proteins for different applications relating to motor proteins.

Publication types

  • Review

MeSH terms

  • Animals
  • Fluorescent Dyes / metabolism*
  • Humans
  • Luminescent Measurements
  • Luminescent Proteins / metabolism
  • Molecular Motor Proteins / metabolism*
  • Molecular Probe Techniques*
  • Nucleic Acids / metabolism
  • Optical Imaging / methods*
  • Quantum Dots

Substances

  • Fluorescent Dyes
  • Luminescent Proteins
  • Molecular Motor Proteins
  • Nucleic Acids