The 86-kilodalton antigen from Schistosoma mansoni is a heat-shock protein homologous to yeast HSP-90

Mol Biochem Parasitol. 1989 Aug;36(1):19-28. doi: 10.1016/0166-6851(89)90196-5.

Abstract

We report the sequence of a cDNA clone encoding an 86-kDa polypeptide antigen (p86) from Schistosoma mansoni. Fusion proteins made in Escherichia coli are recognized by human infection sera. The reading frame of this antigen is highly homologous to those of the large heat-shock proteins of Saccharomyces cerevisiae (HSP90) and Drosophila melanogaster (HSP83). mRNA encoding p86 increases in response to heat shock of adult worms, as does HSP70. Comparisons of the sequences of HSP70 and HSP83 homologues show that these two families of heat-shock proteins are not significantly related except for the last four amino acid residues, which are Glu-Glu-Val-Asp in every case. This sequence is not found at the carboxy terminus of any other protein in the current databases.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Helminth / genetics*
  • Base Sequence
  • Biomphalaria / parasitology
  • DNA / genetics
  • Drosophila melanogaster / genetics
  • Female
  • Heat-Shock Proteins / biosynthesis
  • Heat-Shock Proteins / genetics*
  • Heat-Shock Proteins / immunology
  • Immunoblotting
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Molecular Weight
  • Peptide Fragments / biosynthesis
  • Peptide Fragments / genetics
  • Saccharomyces cerevisiae / genetics*
  • Schistosoma mansoni / genetics*
  • Schistosoma mansoni / immunology
  • Sequence Homology, Nucleic Acid

Substances

  • Antigens, Helminth
  • Heat-Shock Proteins
  • Peptide Fragments
  • DNA

Associated data

  • GENBANK/J04017